Resources
Unleashing Single Molecule Proteomics at Scale
A seminar session conducted on December 6, 2022 as part of the HUPO World Congress in Cancun, Mexico
Speakers and topics included in this session:
- Incremental and disruptive advances in proteomics – Ruedi Aebersold, PhD, Professor of Systems Biology, Institute of Molecular Systems Biology in ETH Zurich (IMSB)
- Comprehensive single-molecule proteomics using Protein Identification by Short-epitope Mapping – Parag Mallick, PhD, Founder and Chief Scientist, Nautilus Biotechnology
- Progress toward the analysis of PARP1 protein variants at single-molecule sensitivity – Philip Lorenzi, PhD, Associate Professor, MD Anderson Cancer Center
Delivering Single-Molecule Proteomics at Scale using Protein Identification by Short epitope Mapping
Learn more about our novel single-molecule proteomic analysis approach and the chemistry, instrumentation, and machine learning that enables intact protein identification and quantification at scale.
A theoretical framework for proteome-scale single-molecule protein identification using multi-affinity protein binding reagents
Demonstrates the potential to efficiently decode greater than 95% of the proteome. Demonstrates potential dynamic range of eleven and a half orders of magnitude in plasma, far exceeding the capabilities of other approaches. Details the ability of our platform to work across multiple organisms, critical for translational research.
Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins
Highlights a unique component of Nautilus’ approach that helps us achieve rapid measurement time alongside wide dynamic range. Using ultra-bright labels gives us substantial signal amplification relative to other approaches enabling us to scan a massive number of protein molecules more efficiently than other methods. Articulates how this innovation simplifies the complexity of the Nautilus instrument.
Highly dense and scalable protein arrays for single-molecule studies
Outlines the critical process of depositing single protein molecules uniformly and completely across the 10 billion ‘landing pads’ on the platform’s high quality, hyper-dense, single-molecule protein array. Proprietary technology based on DNA nanostructures enables single-molecule pad occupancy of over 90% while simultaneously limiting co-localizations. High sensitivity and dynamic range are achieved through high-speed analysis of complex protein samples with hyper-dense single protein molecule arrays, where there are almost never two proteins on the same pad.
