Quantifying proteins and proteoforms with Iterative Mapping on the Nautilus Platform

Iterative Mapping is Nautilus’ novel single-molecule method for quantifying proteins and proteoforms across the full proteome. In the method, billions of protein molecules are captured and displayed on massive, single-molecule arrays – one protein per optically distinguishable landing pad. Fluorescent probes that bind features indicative of protein or proteoform identity are sequentially added to the array, and multiple probe-protein binding events are recorded for every individual protein molecule. After tens to hundreds of unique probe binding cycles, machine learning-powered algorithms convert the probe binding patterns into confident protein and proteoform identifications that are summed for robust quantification.

Join the Iterative Mapping Early Access Program to be one the first to use the Nautilus Platform in your research.

Chapters

00:00 – 00:19 – Introduction

00:20 – 00:38 – Sample preparation and flow cell loading

00:39 – 01:26 – Iterative Mapping process

01:27 – 02:43 – Key applications of Iterative Mapping: Broadscale Proteomics and Targeted Proteoform Studies

02:44 – End – Benefits of Iterative Mapping